Description 1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 1. When a feature or capability applies to only certain readers, this exception is noted. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Multimode Readers Comparison Chart. 0 (IBM Corporation, Armonk, NY, USA). clicked with the SpectraMax L Microplate Reader. With optimized reagents, option to operate the readers. A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. Table 1. There were 12 replicate wells per dilution in both 96-well and 384- well plates. . edu) to be added as a trained user in iLab. . 5. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidePlace the plate into a SpectraMax M5 e plate reader (Molecular Devices). Spectral Range. SpectraMax M5, Analyst HT or Analyst GT. The following document is intended to demonstrate setup of this instrument. 303 unable to cal dark current. 1). Microplate Readers include the M3, M4,. A wireless light sensor (701-999, PS-3212) was purchased from PASCO. . In this product video, see how the SpectraMax M5 quickly reads samples. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. The excitation and the emission. Sale. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Select the fluorescence option and read the total fluorescence. M5. , ~488 nm), has the optical. 1. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Open the Softmax Pro software and select the FP option. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. view more . Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The drawing shows a standard microplate of 15 mm height covered with a lid. Refer to “Installing Detection Cartridges” in. UV/Vis Photometric Performance:. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the. The chamber. . The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 4. type Clear. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Description 1. Fluorescence spectra and UV–vis spectra were recorded on a SpectraMax ® M5 multi-mode microplate reader (Molecular Devices). 72 MB. e. While they play an important role in generating data from a wide range of. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Fluorescent Polarization: Solid black plates, as above. 1. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. 314 low lamp level warning. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. SpectraMax M3, M4, and M5/M5e readers. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataSpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. The SoftMax® Touch Software is installed in. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. . Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Published Dec 16, 2020 | Updated Dec 16, 2020. 2. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Add to Cart. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. 4. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. D 1. . Five preferred detection modes. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Spectral Range. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). 1. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. . Turn on the microplate reader. Control Panel The control panel houses the instrument status LEDs and the microplate. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Step 4: Incubate at room temperature for 1 hour, protected from light. Wide range of applications. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Optimal instrument settings are the same for all three readers. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. Please Inquire. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. ® reader to the computer. . Cables. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. . NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. free tracer decreases, resulting in an. The following document is intended to demonstrate setup of this instrument. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. , ~488 nm), has the optical. For example, microplates containing different samp les can be read using the same or Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. The excitation and the emission wavelengths on. Step 3. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 5. KeySpan adapter for SoftMax Pro and SpectraMax readers. Last lab had ours for years and never had a problem with it. Find regional support. Data Acquisition & Analysis Software. . Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Wavelength Range. 2. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. We would like to show you a description here but the site won’t allow us. 5. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Description. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Bookings are free, but you will need to use iLab to book. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. no. The chamber. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. used. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. . Set the excitation at 531 nm and emission at 595 nm. Fluorescence Microplate Reader Comparison Chart. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Table of Contents. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. . $2,264. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. The KeySpan USA-19HS is often required with new Windows 7. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 26 5039228D Note. Description 1. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 6. Monday to Friday 8am - 5pm GMT. 305 reference level saturation. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 3: The cuvette chamber. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Published Dec 16, 2020 | Updated Dec 16, 2020. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. The dual monochromator optics allow the widest range of applications to be utilized for bior. 3: The cuvette chamber. 313 reference gain check fail. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. 7. l. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. TR-FRET assay. . The chamber. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Category 2 and 3 microplate readers use gradient heating as well. Absorbance measurements of ADMA were acquired using a Biotek Cytation 5-plate reader. 3: The cuvette chamber. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 01) + 0. The . Download PDF versions of the SpectraMax M5 User Guides from this page. For wavelengths in the UV range above 220 nm,. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. In addition, the SpectraMaxMolecular Devices. . Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. com A. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Table of Contents. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Description 1. 4. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 5. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 00 USD. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Read fluorescence polarization at room temperature (RT, 19–25°C). The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. 305 reference level saturation. 2. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. In the M2, M5 and Plus 384, the light. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. D 47 7. . SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. . ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. The dual monochromator optics allow. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Select the fluorescence option and read the total fluorescence. 0 (GraphPad Software, La Jolla, CA, USA) and SPSS version 23. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. 4. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. . The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. HEALTH AND SAFETY CONSIDERAIONS The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Optimized for fluorescence microplate readers. ItSpectraMax® M5多功能读板机为使用者提供了除化学发光以外的其他检测方法，例如：光吸收，荧光强度，荧光偏振和时间分辨荧光。 SpectraMax® L和SpectraMax® M5酶标仪均用SoftMax® Pro软件进行操控和设置，该软件含有CellTiter-Glo检测模板和其他120多个分析模板，这些. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. For SpectraMax L or LMax II, LMax II. . ) Turn on the SpectraMax L and connect the computer to the SpectraMax L using the USB cable. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. D 1. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. . Step 4: Incubate at room temperature for 1 hour, protected from light. Detection modalities are shown in Table 1-1. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. Published Dec 16, 2020 | Updated Dec 16, 2020. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. No accessories are needed and SoftMax® Pro. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Offer Ends In: 18 Days 19: 38: 21. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. Softmax Pro GxP Software 6. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. I have been using the SpectraMax M5 since 2011. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). e. Cavicide (Warehouse, Cat # 79300360) 6. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. Also refer to this Multimode Reader Comparison Chart. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . Samples were measured together with a corresponding reference solvent contained in a. Five preferred modes for diverse bioresearch applications. For very concentrated samples, use a minimum2. The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. 005) Photometric PrecisionThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. UV/Vis Photometric Performance:. 1 or greater versions. 2. When the correct COM port is selected, the. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. Molecular Devices ®(MD) SpectraMax 340PC reader MD VersaMax™ Tunable Microplate readers MD SpectraMax® L Microplate Luminometers MD SpectraMax® M5 systems MD ®SpectraMax 96/384-well plate reader Gemini fluorescence plate reader Automation Hamilton Microlab® STAR™ liquid handling systemThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. Italic and boldface type are used for emphasis. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. 40266 Email: drugdiscoverytech@lifetech. In the M2, M5 and Plus. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. . Download PDF versions of the SpectraMax M5 User Guides from this page. Description 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. Plate chamber design of the SpectraMax category 1 microplate readers. The Spectr. 1. Offer Ends In: 18 Days 19: 38: 21. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. +44-118-944-8000. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Description. Cavicide (Warehouse, Cat # 79300360) 6. Softmax Pro GxP Software 6. Examples: “Press carefully to engage,” “ Do not press down. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. DOCUMENTATION. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Detection of pro-inflammatory factors. Other technologies such as imaging. All reagents were brought to room temperature before use. 00 USD. Step 4. 1) SpectraMax Glo Steady Luc Reporter Assay (6. 303 unable to cal dark current. The dynamic range of the assay was approximately 5 orders of magnitude. 9000-0756. Maximum signal with lowest background. Refurbished. 0 out of 5 $ 17,999 $ 16,995. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. . For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Microplate Reader Shelf. Flexible for 6-well to 384-well microplates. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. 1. Our absorbance plate readers feature our PathCheck Sensor technology and. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Examples: “Press carefully to engage,” “ Do not. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 190 - 1100 nm. ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Sensitive fluorescent quantitation of DNA with the Quant-iT. Features. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY. In this. Table of Contents. Wavelength Range. Figure 4: Transcreener ADP. 2. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Plate chamber design of the SpectraMax category 1 microplate readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. 4. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. 0 mL). Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Methods Preparation of reagents and standards. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. HTRF-compatible SpectraMax Multi-Mode Readers.